Plant Transcription Factor Database
Previous version: v3.0
Oryza sativa subsp. japonica
HSF Family
Species TF ID Description
LOC_Os01g39020.1HSF family protein
LOC_Os01g43590.1HSF family protein
LOC_Os01g43590.2HSF family protein
LOC_Os01g53220.1HSF family protein
LOC_Os01g54550.1HSF family protein
LOC_Os02g13800.1HSF family protein
LOC_Os02g29340.1HSF family protein
LOC_Os02g32590.1HSF family protein
LOC_Os02g32590.2HSF family protein
LOC_Os03g06630.1HSF family protein
LOC_Os03g06630.2HSF family protein
LOC_Os03g12370.1HSF family protein
LOC_Os03g12370.2HSF family protein
LOC_Os03g12370.3HSF family protein
LOC_Os03g25120.1HSF family protein
LOC_Os03g53340.1HSF family protein
LOC_Os03g53340.2HSF family protein
LOC_Os03g53340.3HSF family protein
LOC_Os03g53340.6HSF family protein
LOC_Os03g58160.1HSF family protein
LOC_Os03g58160.2HSF family protein
LOC_Os03g63750.1HSF family protein
LOC_Os04g48030.1HSF family protein
LOC_Os05g45410.1HSF family protein
LOC_Os06g35960.1HSF family protein
LOC_Os06g36930.1HSF family protein
LOC_Os07g08140.1HSF family protein
LOC_Os07g44690.1HSF family protein
LOC_Os08g36700.1HSF family protein
LOC_Os08g43334.1HSF family protein
LOC_Os08g43334.2HSF family protein
LOC_Os09g28200.1HSF family protein
LOC_Os09g28354.1HSF family protein
LOC_Os09g35790.1HSF family protein
LOC_Os09g35790.2HSF family protein
LOC_Os09g35790.3HSF family protein
LOC_Os10g28340.1HSF family protein
LOC_Os10g28340.5HSF family protein
HSF Family Introduction

Heat stress transcription factors (Hsfs) are the major regulators of the plant heat stress (hs) response. Sequencing of the Arabidopsis genome revealed the existence of 21 open-reading frames (ORFs) encoding putative Hsfs assigned to classes A-C. Here we present results of a functional genomics approach to the Arabidopsis Hsf family focused on the analysis of their C-terminal domains (CTDs) harboring conserved modules for their function as transcription factors and their intracellular localization. Using reporter assays in tobacco protoplasts and yeast as well as glutathione-S-transferase (GST) pull-down assays, we demonstrate that short peptide motifs enriched with aromatic and large hydrophobic amino acid (aa) residues embedded in an acidic surrounding (AHA motifs) are essential for transcriptional activity of class A Hsfs. In contrast to this, class B and C Hsfs lack AHA motifs and have no activator function on their own. We also provide evidence for the function of a leucine (Leu)-rich region centered around a conserved QMGΦL motif at the very C-terminus as a nuclear export signal (NES) of class A Hsfs. Sequence comparison indicates that the combination of a C-terminal AHA motif with the consensus sequence FWxxF/L,F/I/L as well as the adjacent NES represents a signature domain for plant class A Hsfs, which allowed to identify more than 60 new Hsfs from the expressed sequence tag (EST) database.

Kotak S, Port M, Ganguli A, Bicker F, von Koskull-Doring P.
Characterization of C-terminal domains of Arabidopsis heat stress transcription factors (Hsfs) and identification of a new signature combination of plant class A Hsfs with AHA and NES motifs essential for activator function and intracellular localization.
Plant J, 2004. 39(1): p. 98-112.
PMID: 15200645